WP12
Tissue-engineered esophageal patch (TEEP)
The reconstruction of the upper alimentary tract after the treatment of esophageal pathologies such as caustic lesions, atresia, perforations and tumors, often requires the use of other organs as substitutes (usually stomach or colon) that are transposed or self-implanted in a different anatomical site.These complex surgical techniques are burdened by high morbidity and mortality even if the long term functional results are sub-optimal.Tissue engineering is proving to be an interesting promise for the development of tissue or organ substitutes, such as the esophagus, that can overcome the current limits of surgery; in fact, clinical applications in urology and cardiac have already been described.Based on these considerations, this project aims to develop an esophageal substitute with tissue engineering techniques and to develop implantation techniques for a clinical application.
TEAM
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S. Merigliano
Specialist in General Surgery. Full Professor of General Surgery. Director of the Surgical Clinic 3 of the University of Padua. President of the School of Medicine of the University of Padua. He is the holder of the teaching of General Surgery II. Teacher of surgical techniques at the Specialization Schools in General Surgery, Thoracic Surgery and Vascular Surgery of the University of Padua. He has over4,000 general surgery, esophagus, stomach and colon surgery and proctology with both traditional and laparoscopic techniques.
M. Valmasoni
Specialist in General Surgery and Doctor of Philosophyin Surgical Sciences and Technological Applications, Medical Director at the Hospital of Padua. He is dedicated to Epatobiliopancreatic Surgery and Transplantation and to EsophagealSurgery. He deals with general surgery and oncological surgery of the esophagus and stomach, hepatobiliopancreatic surgery and laparoscopic surgery. He has over 2000 major surgery operations. He is a contract professor and tutor at the School of Specialization in General Surgery and Professor at the Doctoral School in Clinical and Experimental Medical Sciences of the University of Padua. He is a fellow of the American College of Surgeon and a member of many national and international scientific societies, including the International Society for the Diseases of Esophagus (ISDE) and the Society for Surgery of the Alimentary Tract (SSAT). He is a reviewer for several international scientific journals.
S. Barbon
Ph.D. in Biology and Regenerative Medicine, specialization in Tissue Engineering and Transplantation (2010/2012 - University of Padova). Master Degree in Evolutionary Biology (2007/2009 - University of Padova) and Bachelor Degree in Biology (2004/2007 - University of Padova). From 2013 to 2014, she has been a Post-doc Researcher at T.E.S. Foundation, Padova. In 2015, she worked as Post-doc Researcher at the Department of Molecular Medicine, University of Padova. From 2016 to 2018, she worked as Post-doc Researcher at the Department of Pharmaceutical and Pharmacological Sciences, University of Padova. Since March 2019, she works as Post-doc Researcher et the Department of Neurosciences, University of Padova, in the L.i.f.e.L.a.b. Program of CORIS Veneto. Her scientific activity mainly deals with research programs which combine specific biological science expertises with tissue engineering and regenerative medicine issues with the aim of obtaining biological substitutes that replace or maintain the functionality of human tissues. The experimental work involves the isolation and culture of cells (primary cultures of tissue specific cells and stem cells; immortalized cell lines), studies on growth factors and stem cell differentiation, molecular biology analyses for gene and protein expression, fabrication and repopulation of scaffolds of natural (platelet-rich products, biological matrices) and synthetic (polyvinyl alcohol-based hydrogels) origin and in vivo implantation of the fabricated scaffolds into animal models of tissue damage.
A. Biccari
Bachelor in Biotechnology at University of Teramo (2014-2017) and Master Degree in Pharmaceutical, Veterinary and Medical Biotechnologies at University of Parma (2017-2019). During his Master degree, he worked for culture cells (primary culture such as organoids from patients or immortalised tumor cell line), decellularization and repopulation of human biological matrix (Colon-rectum and pancreas) and synthetic (hydrogel), Immunofluorescence and Immunohistochemistry analyses, Scanning Electron Microscope, DNA and RNA quantification, Structural matrix protein quantification (Collagen and Glycosaminoglycan). From december 2019, he started to work for Discog, University of Padova, in the LifeLab Program of CORIS Veneto (“Tissue-engineered esophageal patch (TEEP). From allogenic tissue harvesting to engineered patch clinical use”.)
M. Costantini
Born in 1955, he graduated in Medicine and Surgery in Padua in 1980 with honors. Specialized in Padua in General Surgery (1985) and in Gastroenterology (1989), he has always been involved in diagnostics and minimally invasive therapy of esophageal-gastric functional diseases. In 1991-92 he was a Research Fellow at the University of Southern California, Los Angeles (Prof. T.R. DeMeester). He is currently responsible for the Simple Unit of Esophagus-Gastric Physiopathology and the Laboratory of Digestive Physiopathology attached to the Surgical Clinic III of the University -Hospital of Padua.
R. Salvador
Graduated in Medicine and Surgery at the University of Padua in 2005. He specialized in General Surgery in Padua with honors; he has always dealt with esophageal-gastric diseases. He is currently a researcher at the University of Padua.In 2007-2008 he was an Esophageal Research Fellow at the University of Rochester Medical Center, Rochester NY, USA (prof. J. H. Peters). He is a member of many national and international scientific societies. including the International Society for the Diseases of Esophagus (ISDE) and the Society for Surgery of the Alimentary Tract (SSAT). He is a reviewer for several international scientific journals.Member of SICPRE (Italian Society of Plastic Reconstructive and Aesthetic Surgery), SICM (Italian Society of Hand Surgery) and EACA (European Society of Clinical Anatomy); member of the Centre for Mechanics of Biological Materials (CMBM) at the University of Padova. Speaker in International and National Scientific Congresses.
E. Sefora Pierobon
Specialist in General Surgery and Research Doctor, Medical Director at the Hospital of Padua. She graduated in Medicine and Surgery and specialized in General Surgery in Padua. He received his PhD in Clinical and Experimental Sciences in 2018 in Padua. It is dedicated to Kidney and Pancreatic Transplantation Surgery and to Esophageal Surgery. In 2013-2014 she was Visiting Physician at the Center for Hepatobiliary Surgery and Multivisceral Transplantation at Mount Sinai Hospital in New York.She was Medical Director at the General Surgery with Thoracic Activity of the Ca ’Foncello Hospital in Treviso and is currently Medical Director at the University of Padua Hospital.