Impact of naïve and engineered extracellular vesicles on the viability of hearts donated after circulatory death: study in a pig model.
Investigation in a pig model the effects of naïve EVs and of EV- SerpinB3 supplementation in the reperfusion solution in hearts explanted after circulatory death.
Graduated in Medicine and Surgery at the University of Padua, in 1980. In 1985 she achieved the Specialization in Internal Medicine and in 1988 in Clinical and Laboratory Haematology.In 1989 Assistant Physician at the Medical Clinic II, University of Padua. In 2006 Associate Professor of Internal Medicine at the University of PaduaIn 2013 National Habilitation for Full Professor in Internal Medicine and in Gastroenterology.Past member of the Board of the Italian Association for the Study of the Liver (AISF) and past AISF Secretary (2000-2002).From 2006 to 2015 member of the Scientific Board of the Centre of Experimental Surgery, University of Padua and Director of that Center from October 2015.Responsible for the Molecular Hepatology Laboratory (since 2004). Responsible for the Liver Biobank, approved by the localEthics Committee (since 2007).MAIN LINES OF RESEARCHProf. Pontisso has conducted research in molecular biology and in novel recombinant HBV vaccines at Pasteur Institute, Paris. At the Rush University in Chicago she carried out studies on mechanisms of hepatitis B virus infection. Further studies led to the identification of the serine protease inhibitor SerpinB3, a pleiotropic moleculeinvolved in several biological activities. At present the main research projects are focused on the mechanisms of tissue repair, liverdisease progression, carcinogenesis and on the development of new prognostic markers for primary liver tumors.
Long-term experience in clinical research and basic biomedical research.Extended experience in the standardization of immunoenzimatic assay (ELISA, Dot blot)for the identification of serum circulating biomarkers. Good experience with electrophoretic and chromatographic methods (affinity, ion exchange, gel filtration).Basic experience with cellular and molecular biology tecniques includingRNA, DNA extraction recombinant protein production, PCR and polymorphisms analysis.Ability to work well in a team as well as independently.
Characterization of thecellular receptorforthehepatitis B virusthat ledto isolationofa protein that bindsthe virus itself(SCCA), the studyofexpression of this protein inhepatocellular carcinomasanditspotentialinvolvement in the developmentof hepatocellular carcinoma. In 2002the studyofgenomic sequencesspecificforsquamous cellcarcinomaantigen(SCCA) onhepatictumor tissuethroughautomated sequencingled to the identificationof a SCCA-variantcalledSCCA-PD, placed in GenBank.Carcinogenesis and new biomarkers as hepatological diagnostic and prognostic factors is the main topic of my research.
Long-term experience in basic biomedical research.Extended experience with cellular and molecular biology techniques including cell transfection, plasmid production, Real Time PCR, microarray, Western blotting, Elisa test, immunofluorescence microscopy, recombinant protein production, polymorphisms analysis by High-Resolution Melting , NGS.