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Impact of naïve and engineered extracellular vesicles on the viability of hearts donated after circulatory death: study in a pig model.

Investigation in a pig model the effects of naïve EVs and of EV- SerpinB3 supplementation in the reperfusion solution in hearts explanted after circulatory death.

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M. Muraca

Present position: Associate Professor of Medicine, Dept. of Women’s and Children’s Health, University of Padova (Italy) 
Education: M.D. (University of Padova) PhD (University of Leuven, Belgium), Specialist in Internal Medicine and in Gastroenterology (University of Padova) 
Head of Clinical Chemistry and Microbiology, Responsible for the Research Area in Regenerative Medicine/Cell Factory, Bambino Gesù Research Hospital, Rome (2004-2014) 
Qualified Person for the production of Advanced Therapy Medical Products (Italian Medicines Agency AIFA,2007). 
Expert in Advanced Therapies (clinical) at the European Medicines Agency (2011-present).
Author and co-author of 154 original articles in peer-reviewed international journals, 3 international books in English, 2 books in Italian, 16 chapters in international books, 51 original articles and book chapters in Italian. 
He participated in the first international clinical trial on the treatment of liver failure with a bioartificial liver device (Ann Surg. 2004; 239:660) and coordinated the first clinical hepatocyte transplantation in Europe (Lancet 2002; 359: 317). 
He is inventor of four patents/patent proposals in cell therapy. 
Awards: 10/11/2006: International Award “Giuseppe Sciacca”, section Medicine; 18/07/2011: Award “Gentlemen d’Italia” for scientific activity.


P. Pontisso

Graduated in Medicine and Surgery at the University of Padua, in 1980. In 1985 she achieved the Specialization in Internal Medicine and in 1988 in Clinical and Laboratory Haematology.In 1989 Assistant Physician at the Medical Clinic II, University of Padua. In 2006 Associate Professor of Internal Medicine at the University of PaduaIn 2013 National Habilitation for Full Professor in Internal Medicine and in Gastroenterology.Past member of the Board of the Italian Association for the Study of the Liver (AISF) and past AISF Secretary (2000-2002).From 2006 to 2015 member of the Scientific Board of the Centre of Experimental Surgery, University of Padua and Director of that Center from October 2015.Responsible for the Molecular Hepatology Laboratory (since 2004). Responsible for the Liver Biobank, approved by the localEthics Committee (since 2007).MAIN LINES OF RESEARCHProf. Pontisso has conducted research in molecular biology and in novel recombinant HBV vaccines at Pasteur Institute, Paris. At the Rush University in Chicago she carried out studies on mechanisms of hepatitis B virus infection. Further studies led to the identification of the serine protease inhibitor SerpinB3, a pleiotropic moleculeinvolved in several biological activities. At present the main research projects are focused on the mechanisms of tissue repair, liverdisease progression, carcinogenesis and on the development of new prognostic markers for primary liver tumors.


A. Tolomeo

Anna Maria Tolomeo is Biologist at the University of Padova. Graduated in Evolutionary Biology at the University of Padua (Italy) in 2014, Anna Maria earned the Ph.D. in Developmental Medicine at the University of Padova in 2018, supervised by Prof. Maurizio Muraca and co-supervised by Prof. Nicola Elvassore. Anna Maria focused her doctoral study on two different topics, the first on the evaluation of immunomodulatory effects of extracellular vesicles (EVs) in an in-vivo model of inflammatory bowel diseases learning to control EVs’ isolation procedures, analytical techniques and animal handling. The second PhD topic on the integration of transcriptional programming and micro-technologies for in-vitro modelling of neural development. During these studies she visited the Prof. Giuseppe Testa Lab (Milan, Italy) where she focused on the rapid conversion of hiPSCs into neurons and the Prof. Zaal Kokaia Lab (Lund, Sweden) for the in vitro neural progenitors’ generation. Anna Maria joined the Cellular Engineering group in April 2019 as a Post-doctoral Researcher under the LifeLab project. She is currently developing a highly specialized clinical platform based on pig model and a high-throughput technological platform based on organ-on-chip model both for the recovery, reconditioning and preservation of organs harvested after circulatory death.


G. De Lazzari

PhD student at the Department of Women’s and Children’s Health, University of Padova. She obtained the Bachelor’s degree in Molecular Biology (2016) with the thesis: “Leuchine-rich repeat kinase 2 (LRRK2) pharmacological inhibition abates α-synuclein gene-induced neurodegeneration” and a Master’s degree in Biomedical science (2018) with the thesis: “Inhibition of JAK2/STAT3 axis leads to apoptosis of Chronic Lymphocytic Leukemia neoplastic clone”. She is currently working on her PhD project: “Development of ex-vivo culture methods for Extracellular Vesicles testing” (2019-). In this first year she gained experience in evaluate the immunomodulatory effect of Extracellular Vesicles in both in vitro and in vivo disease models.


R. Malvicini

Biochemist, graduated from the University of Buenos Aires (Argentina). From 2017, he is a PhD student at the Institute for Traslational Medicine, Transplantation and Bioingeneering (IMeTTyB-CONICET-Favaloro Fundation); he is funded by CONICET, Argentina’s largest science institution. He works in the Gene regulation and stem cells laboratory, directed by Prof. Gustavo Yannarelli. His doctorate proyect is about isolation and characterization of human Mesenchymal stem/stromal cells-derived Extracellular Vesicles and their use in regenerative medicine, in particular, acute myocardial infarction. Also, he is a teacher at the department of compared molecular and cellular biology at the Favaloro University. At the moment, he is carrying out an intership at the laboratory of Regenerative Medicine, under the LifeLab project, supervised from Prof. Maurizio Muraca, thanks to a scholarship from the Italo-latinamerican institute (IILA) and the University of Padova. His work regards the developing of functional assays to assess extracellular vesicles’ anti-inflammatory activity and, also, the evaluation of the effect of the extracellular vesicles on the recovery and preservation of pig hearts harvested after circulatory death.


A. Biasiolo

Long-term experience in clinical research and basic biomedical research.Extended experience in the standardization of immunoenzimatic assay (ELISA, Dot blot)for the identification of serum circulating biomarkers. Good experience with electrophoretic and chromatographic methods (affinity, ion exchange, gel filtration).Basic experience with cellular and molecular biology tecniques includingRNA, DNA extraction recombinant protein production, PCR and polymorphisms analysis.Ability to work well in a team as well as independently.


M. Ruvoletto

Characterization of thecellular receptorforthehepatitis B virusthat ledto isolationofa protein that bindsthe virus itself(SCCA), the studyofexpression of this protein inhepatocellular carcinomasanditspotentialinvolvement in the developmentof hepatocellular carcinoma. In 2002the studyofgenomic sequencesspecificforsquamous cellcarcinomaantigen(SCCA) onhepatictumor tissuethroughautomated sequencingled to the identificationof a SCCA-variantcalledSCCA-PD, placed in GenBank.Carcinogenesis and new biomarkers as hepatological diagnostic and prognostic factors is the main topic of my research.

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