©2019 by APC.

WP20

Optimization of a Multi-Photon Micrroscope for in-vivo Label-Free STED imaging

WP20 aims to provide to the othergroupsof the programmethe state of the art of microscopy and nanoscopy. Microscopy is, in fact, the main investigative tool necessary to monitor the regenerative processes of the different tissues and organs, which are the main objective of the LIFELAB project.In particular, we will use fluorescence multiphoton microscopy (MPM) which is a non-invasive technique to obtain images from tissues or in live animals. MPM exploits the absorption in a single quantum event of two photons by specific molecular receptors, allowing characterization of the basic processes of recellularization. We will monitor the differentiation of cells (eg pluripotent stem cells in cardiac cells) step-by-step by mapping intracellular morphology (eg sarcomere formation, distribution of mitochondria, ...) over large areas.This technique has been specialized in a super-resolution configuration, developing spatial and temporal coherence techniques of two laser sources, and lenseswithout aberrations

 

TEAM

Associate professor at the Department of Physics and Astronomy “Galileo Galilei” of Padua University.

Gianluca Ruffato

Post-doc researcher at the Department of Physics and Astronomy “Galileo Galilei” of Padua University.

Giulia Borile

Post-doc researcher at the Institute of Pediatric Research - Città della Speranza of Padua.

Michele Massari

Senior post doc researcher at DFA (Dep of Physics and Astronomy) LIFE LAB funded.

Pietro Capaldo

Post-doc researcher at the Italian National Research Council (CNR) of Trieste, Institute of Materials (IOM).