WP20

Optimization of a Multi-Photon Micrroscope for in-vivo Label-Free STED imaging

WP20 aims to provide to the othergroupsof the programmethe state of the art of microscopy and nanoscopy. Microscopy is, in fact, the main investigative tool necessary to monitor the regenerative processes of the different tissues and organs, which are the main objective of the LIFELAB project.In particular, we will use fluorescence multiphoton microscopy (MPM) which is a non-invasive technique to obtain images from tissues or in live animals. MPM exploits the absorption in a single quantum event of two photons by specific molecular receptors, allowing characterization of the basic processes of recellularization. We will monitor the differentiation of cells (eg pluripotent stem cells in cardiac cells) step-by-step by mapping intracellular morphology (eg sarcomere formation, distribution of mitochondria, ...) over large areas.This technique has been specialized in a super-resolution configuration, developing spatial and temporal coherence techniques of two laser sources, and lenseswithout aberrations

 

TEAM

Associate professor at the Department of Physics and Astronomy “Galileo Galilei” of Padua University.

Gianluca Ruffato

Post-doc researcher at the Department of Physics and Astronomy “Galileo Galilei” of Padua University.

Dott.ssa Giulia Borile

Dr. Giulia Borile started to work on advanced microscopy and biophysics in Paris at ESPCI, under the supervision of 
Prof. Dubertret in 2010, working on structured illumination microscopy for quantum dots tracking. After her graduation in Physics, she has been granted of a PhD Fellowship at the School of Biosciences and Biotechnologies. She embraced a challenging project aimed at investigating the role of the cardiac calcium release channels in the modulation of Ca2+ homeostasis in the heart, using multiphoton microscopy. As a part of her PhD, she was also involved in a project that aimed at studying the tissue requirements for cardiac arrhythmias with optogenetic approach. Dr. Borile then moved in the lab of Prof. Romanato, where she started to develop and optimize multiphoton microscope techniques for biomedical applications. She provided preliminary data for LIFELAB project and is now enlarging the collaborations with all WPs in LIFELAB program. Dr. Borile has been awarded from Institute of Pediatric Research of a grant that allowed her to spend 9 months as visiting scientist at Francis Crick Institute in London in Advanced Microscopy Lab. of Dr. Kurt Anderson.

Michele Massari

Senior post doc researcher at DFA (Dep of Physics and Astronomy) LIFE LAB funded.

Pietro Capaldo

Post-doc researcher at the Italian National Research Council (CNR) of Trieste, Institute of Materials (IOM).

Dott.ssa Deborah Sandrin

Dr. Deborah Sandrin in 2017 obtained the PhD degree in the molecular physical chemistry group of Prof. C. Seidel at the Universität of Düsseldorf (Germany) followed by a post-doc position in the group of Prof. J.C. Scaiano at the University of Ottawa (Canada). In the group of Prof. C. Seidel, she learned single-molecule fluorescence techniques using a multiparameter fluorescence image spectroscopy (MFIS) approach, an important tool in fluorescence imaging that records multiple fluorescence parameters simultaneously with picoseconds accuracy over time periods of hours. She used MFIS to gain insight into the fundamental processes determining the motion of macromolecules in polymeric matrices, the dynamical hindrance of polymeric dextran molecules diffusing as probe through a polyacrylamide hydrogel was explored. 
In the group of Prof. J.C. Scaiano (2018-2020) she had the possibility to extend such knowledge by studying the palladium catalysed Suzuki-Miyaura reaction, one of the most important methods for C–C cross coupling, in real-time with high spatial resolution microscopy by using Total Internal Reflection Microscopy (TIRFM) and Stochastic Optical Reconstruction Microscopy Imaging (STORM). The outcome showed the strength of single molecule studies unveiling fundamental mechanisms in heterogeneously catalysed reactions, otherwise difficult to explore with classical ensemble experiments. 
In 2020 she joined LIFELAB program in the WP 20 to implement super-resolution microscopy and develop fluorophores.

 

Via Nicolò Giustiniani, 2
35128 Padova PD

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