WP6

Decellularized human skeletal muscle as biologic scaffold for reconstructive surgery.

Loss of skeletal muscle tissues may occur as a result of traumatic injuries, degenerative changes, infections or neoplasms. Clinically, restoration of large volumetric muscle loss involves transfer of autologous muscle tissue or muscle flaps from local or distant sites to the area of injury, although these procedures are associated with donor site morbidity, resulting in functional loss and volume deficiency at the donor site. Therefore, the development of engineered skeletal muscle grafts from homologous and autologous tissues has recently been proposed as a potential solution for replacement of volumetric muscle losses, in order to avoid donor site complications and permit the availability of large quantities of tissues with good histocompatibility. In this context, the present study aims at standardizing a protocol to decellularize human diaphragm as a novel and still poorly investigated source for the development of decellularized biological scaffolds for skeletal muscle regeneration. The resulting acellular matrix will undergo biomechanical characterization and re-populated by the use of a bioreactor to mimic physiological pressure, flow, and environmental conditions.

 
 

TEAM

F. Bassetto

Medical Degree (1984 - University of Padova), specialized in Plastic, Reconstructive and Aesthetic Surgery (1984 - University of Padova) and in Hand Surgery (2004 – University of Florence). Attending Physician in the department of Plastic, Reconstructive and Aesthetic Surgery (1992-1998, University of Padova); Researcher in the Department of Plastic, Reconstructive and Aesthetic Surgery (1998-2005, University of Padova); Associate Professor of Plastic, Reconstructive and Aesthetic Surgery (2005-2008, University of Padova); Director of the Residency Program in Plastic, Reconstructive and Aesthetic Surgery (2005-2010, University of Padova); Director of the Department of Plastic, Reconstructive and Aesthetic Surgery (2012-Today University of Padova); Full-Professor of Plastic, Reconstructive and Aesthetic Surgery (from January 2014-Today, University of Padova). Other positions: Director of the II° degree Master in Plastic, Reconstructive and Aesthetic Surgery (since 2001-Today, University of Padova). Author of more than 190 original works in extenso, speaker at numerous National and International Congresses. Responsible of 62 research projects. Member of  the Italian society of plastic, reconstructive and aesthetic surgery (SICPRE); Member of the  European Workshop of Advanced Plastic Surgery; Member of the International Confederation of Plastic, Reconstructive and Aesthetic Surgery; Member of the European Burns Association; Member of the International Society for Burn Injures; Member of the European Tissue Repair Society; Member of the Scar Club.

V. Vindigni

Medical Degree (1997, University of Padova), specialized in Plastic, Reconstructive and Aesthetic Surgery (2002, University of Padova). Ph.D. in Tissue and Transplants Engineering (2005, University of Padova); Research scholarship in the histology, microbiology and medical biotechnologies department, in the “reconstruction of small arteries” field (2006, University of Padova); Research scholarship in the histology, microbiology and medical biotechnologies department, in the “in vitro reconstruction of micro vascularized adipose tissue” field (2007, University of Padova); Researcher and Attending Physician in the Department of Plastic, Reconstructive and Aesthetic Surgery (2007- Today, University of Padova); Associate Professor of Plastic, Reconstructive and Aesthetic Surgery (2016- Today); Director of the Residency Program in Plastic, Reconstructive and Aesthetic Surgery (2016-Today); Full-Professor of Plastic, Reconstructive and Aesthetic Surgery (2016-Today, University of Padova). Author of more than 100 original works in extenso, speaker at numerous National and International Congresses. Other positions: Member of the Italian society of plastic, reconstructive and aesthetic surgery (SICPRE); Member of the Italian society of microsurgery (SIM); Member of the Italian society of obesity aesthetic plastic surgery (AICPEO); Member of the European Association of Plastic Surgeons (EURAPS); Member of the American Society of Plastic Surgeons (ASPS); Member of the World Society for Reconstructive Microsurgery (WSRM). International Master’s Degree in Reconstructive Microsurgery (University of Barcelona).

R. De Caro

Medical Degree (1980 - University of Padova), specialized in Pathological Anatomy (1984 - University of Padova) and in Legal Medicine (1987 - University of Trieste). At the University of Padova: Researcher at the Institute of Normal Human Anatomy (1983-1992); Associate Professor of Topographic Anatomy (1992-2000); Full-Professor of Human Anatomy (from October 2000 - Today); Director of the Department of Human Anatomy and Physiology (2004-2011); Director of the Department of Neurosciences (since October 1, 2019). Other positions: President of Courses in Medicine and Surgery (2006-2013) and Physiotherapy (since 2013); Director of the Master in manual therapy and musculoskeletal rehabilitation (since 2012). Director of the Centre for Mechanics of Biological Materials (CMBM) at the University of Padova up to 2019. Regarding Body Donation, Prof. De Caro is the chief of the staff of the “Body Donation Program” of the University of Padova (Reference Centre for Body Donation of the Veneto Region) and of the “Inter-department Centre for Teaching on Cadaver - A. Vesalio”. Author of more than 300 original works in extenso. Member of the editorial board of: Clinical Anatomy, Surgical and Radiological Anatomy, European Journal of Anatomy, Translational Research in Anatomy.
President of the “Teachers board of Human Anatomy”, Member of the Italian Society of Anatomy e Histology, Past President of the European Association of Clinical Anatomy, Corresponding Fellow of the British Association of Clinical Anatomy, Vice-President of the Transeuropean Pedagogic Anatomical Research Group, Member of the Anatomical Society, American Association of Clinical Anatomy, International Society of History of Medicine.
Prof. De Caro personally performed over 5000 cadaveric dissections, for research scientific and educational purposes. Speaker at numerous national and international conferences, including by invitation. Moreover, Prof. De Caro has organized and taught in several dissection workshops in clinical anatomy and courses for surgeons, also in collaboration with other Institutions. Consultant activities in the field of Forensic Clinical Anatomy.

A. Porzionato

Medical Degree (2001 - University of Padova), specialized in “Legal Medicine” (2005 - University of Padova). From October 1, 2005 he is Assistant Professor in Human Anatomy (Institute of Human Anatomy - University of Padova); Ph.D. in Medical, Clinical and Experimental Sciences, Neuroscience curriculum (2009 - University of Padova); from December 1, 2011 he is Associate Professor of Human Anatomy (Institute of Human Anatomy - University of Padova); National Scientific Qualification for Full Professor in Human Anatomy since 2016. Other positions: Member of the Scientific Committee of the Dept. of Molecular Medicine of the University of Padova from 2012-2016; External Reviewer for the Research Grant Council of Hong Kong in 2012; Representative for the School of Medicine in the “Committee for Didactics Quality”; Director of the Course in Aerospace Medicine from 2013-2014; Member of the Ph.D. Program in Molecular Medicine from 2014-2015. Prof. Porzionato is active in many interdisciplinary research projects in the field of regenerative medicine and tissue engineering (development of biological scaffolds and innovative artificial materials), also cooperating with the Centre for Mechanics of Biological Materials (CMBM) at the University of Padova. Other main interests regard experimental models of bronchopulmonary dysplasia, neuroscience (central and peripheral chemoreceptors, neuroanatomy/neuropathology of the brainstem) and clinical anatomy; he is principal investigator of many research projects since 2007. Regarding Body Donation, Prof. Porzionato is member of the staff of the “Body Donation Program” of the University of Padova (Reference Centre for Body Donation of the Veneto Region) and of the “Inter-department Centre for Teaching on Cadaver - A. Vesalio”, also coordinating numerous dissection workshops for surgeons and students of the Medical School. Author of more than 240 in extenso papers with Impact Factor (˃600), speaker at numerous National and International Congresses. Participation in PRIN 2005 and 2007. Head of Research Project of the University of Padova in 2014. Scientific head for Scholarship in Regenerative Medicine and Tissue Engineering (2016 and 2017 from TES - Tissue Engineering and Signaling Foundation onlus). Tutor at numerous workshops of dissection in anatomical and clinical courses for surgeons. Member of the Editorial Board of European Journal of Anatomy, Frontiers in Oncology, Frontiers in Cell and Developmental Biology, Forensic Science and Criminology, Annals of Medical Case Reports - Section: Forensic and Legal Medicine Case Reports. Consultant activities in the field of Forensic Clinical Anatomy.

V. Macchi

Medical degree (1998 - University of Padova), specialized in Radiology (2002 - Dept. of Radiology, University of Padova) and Ph.D. in Medical, Clinical and Experimental Sciences, Neuroscience curriculum (2006 - Dept. of Anatomy and Physiology, University of Padova). She is Associate Professor of Human Anatomy at the University of Padova, since 2010; National Scientific Qualification for Full Professor in Human Anatomy, since 2016. Other positions: Member of the Ph.D. Program in Molecular Medicine from 2014-2015; since 2016, she is member of the Scientific Committee of the Dept. of Neurosciences, University of Padova. Her scientific activity focuses on clinical and radiological anatomy, considering the role of adipose tissue in the onset and development of osteoarthritis.  he is principal investigator of many research projects since 2007. Vice-director of the Centre for Mechanics of Biological Materials (CMBM) at the University of Padova, she is active in interdisciplinary research projects based on regenerative medicine and tissue engineering. Regarding Body Donation, Prof. Macchi is member of the staff of the “Body Donation Program” of the University of Padova (Reference Centre for Body Donation of the Veneto Region) and of the “Inter-department Centre for Teaching on Cadaver – A. Vesalio”. Director of the Specialization Course in Clinical Anatomy of the musculoskeletal system. Moreover, she is also responsible for coordination of numerous dissection workshops for surgeons and students of the Medical School. Author of more than 240 in extenso papers with Impact Factor (˃600). Participation in PRIN 2005 and 2007. Head of Research Project of the University of Padova in 2015. Scientific head for Scholarship in Regenerative Medicine and Tissue Engineering (2016 and 2017 from TES – Tissue Engineering and Signaling Foundation onlus). Associate Editor of anatomical Science Education, Surgical and Radiologic Anatomy, European Journal of Anatomy and Journal of the Anatomical Society of India. Speaker and  invited speaker at numerous National and International Congresses.

F. Facchin

Medical Degree (2013 – University of Padova), specialized in Plastic, Reconstructive and Aesthetic Surgery (2019 – University of Trieste), Master in Aesthetic Plastic Surgery. ECFMG Certified.
PhD student in Molecular Medicine – Curriculum in Regenerative Medicine.
In 2013 he won a grant sponsored by the University of Padova to train in medicine and clinical research at the Columbia University Medical Center. In 2014 he did research observership and training at the wound healing laboratory at the University of Brigham and Women's Hospital Harvard, directed by Prof. D.P. Orgill.
During his training he focused in reconstructive procedures and microsurgery. He is active as Plastic Surgeon in clinical and surgical activity at the Unit of Plastic and Reconstructive Surgery of the University-Hospital of Padova.
The main projects of his PhD program involve the project of muscle decellularization as scaffold for complex reconstruction and breast tissue isolation to study the mechanisms of oncogenesis.
Member of the Italian society of Plastic, Reconstructive and Aesthetic surgery (SICPRE); Member of the  Italian Society of Microsurgery; Member of the International Confederation of Plastic, Reconstructive and Aesthetic Surgery. He has participated as a speaker in numerous congresses and level training courses national and international. In 2014 he won the SICPRE Youth Award and contributed to the work that won the Rossoni prize of the Italian Society of Hand Surgery.

S. Barbon

Ph.D. in Biology and Regenerative Medicine, specialization in Tissue Engineering and Transplantation (2010/2012 - University of Padova). Master Degree in Evolutionary Biology (2007/2009 - University of Padova) and Bachelor Degree in Biology (2004/2007 - University of Padova). From 2013 to 2014, she has been a Post-doc Researcher at T.E.S. Foundation, Padova. In 2015, she worked as Post-doc Researcher at the Department of Molecular Medicine, University of Padova. From 2016 to 2018, she worked as Post-doc Researcher at the Department of Pharmaceutical and Pharmacological Sciences, University of Padova. Since March 2019, she works as Post-doc Researcher et the Department of Neurosciences, University of Padova, in the L.i.f.e.L.a.b. Program of CORIS Veneto. Her scientific activity mainly deals with research programs which combine specific biological science expertises with tissue engineering and regenerative medicine issues with the aim of obtaining biological substitutes that replace or maintain the functionality of human tissues. The experimental work involves the isolation and culture of cells (primary cultures of tissue specific cells and stem cells; immortalized cell lines), studies on growth factors and stem cell differentiation, molecular biology analyses for gene and protein expression, fabrication and repopulation of scaffolds of natural (platelet-rich products, biological matrices) and synthetic (polyvinyl alcohol-based hydrogels) origin and in vivo implantation of the fabricated scaffolds into animal models of tissue damage.

 

Via Nicolò Giustiniani, 2
35128 Padova PD

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