WP7
Leukocyte Fibrin Platelet (LFP) patches for topical anti-inflammatory treatment and tissue regeneration: a pre-clinical characterization.
Within the biomaterial field, platelet-rich fibrin has recently aroused widespread interest as a bioactive scaffold delivering growth factors, cytokines and immune/stem-like cells for immunomodulation and tissue healing purposes. In this context, the Department of Immunohematology and Transfusion Medicine of Belluno hospital standardized a new method to produce an autologous Leukocyte Fibrin Platelet membrane (LFPm) with increased concentrations of the most important elements of tissue regeneration. This adhesive membrane, prepared according to L. 219/2005, is already used for experimental treatment of cartilage lesions, but scarse pre-clinical data supporting its efficacy are currently available. Hence, this project firstly aims at performing a series of in vitro studies to support the rationale for LFP patches clinical use. Moreover, in order to adequate LFPm properties to a wider range of clinical application, this project will also propose to prepare LFPm-derived homogenized extracellular matrix to be combined with a novel polymer based on oxidized polyvinyl alcohol (PVA). These hybrid composites will take advantage of both LFPm content in regenerative elements and PVA mechanical properties.
Team
R. De Caro
Medical Degree (1980 - University of Padova), specialized in Pathological Anatomy (1984 - University of Padova) and in Legal Medicine (1987 - University of Trieste). At the University of Padova: Researcher at the Institute of Normal Human Anatomy (1983-1992); Associate Professor of Topographic Anatomy (1992-2000); Full-Professor of Human Anatomy (from October 2000 - Today); Director of the Department of Human Anatomy and Physiology (2004-2011); Director of the Department of Neurosciences (since October 1, 2019). Other positions: President of Courses in Medicine and Surgery (2006-2013) and Physiotherapy (since 2013); Director of the Master in manual therapy and musculoskeletal rehabilitation (since 2012). Director of the Centre for Mechanics of Biological Materials (CMBM) at the University of Padova up to 2019. Regarding Body Donation, Prof. De Caro is the chief of the staff of the “Body Donation Program” of the University of Padova (Reference Centre for Body Donation of the Veneto Region) and of the “Inter-department Centre for Teaching on Cadaver - A. Vesalio”. Author of more than 300 original works in extenso. Member of the editorial board of: Clinical Anatomy, Surgical and Radiological Anatomy, European Journal of Anatomy, Translational Research in Anatomy.
President of the “Teachers board of Human Anatomy”, Member of the Italian Society of Anatomy e Histology, Past President of the European Association of Clinical Anatomy, Corresponding Fellow of the British Association of Clinical Anatomy, Vice-President of the Transeuropean Pedagogic Anatomical Research Group, Member of the Anatomical Society, American Association of Clinical Anatomy, International Society of History of Medicine.
Prof. De Caro personally performed over 5000 cadaveric dissections, for research scientific and educational purposes. Speaker at numerous national and international conferences, including by invitation. Moreover, Prof. De Caro has organized and taught in several dissection workshops in clinical anatomy and courses for surgeons, also in collaboration with other Institutions. Consultant activities in the field of Forensic Clinical Anatomy.
V. Macchi
Medical degree (1998 - University of Padova), specialized in “Radiology” (2002 - Dept. of Radiology, University of Padova) and Ph.D. in Medical, Clinical and Experimental Sciences, Neuroscience curriculum (2006 - Dept. of Anatomy and Physiology, University of Padova). She is Associate Professor of Human Anatomy at the University of Padova, since 2010; National Scientific Qualification for Full Professor in Human Anatomy, since 2016. Other positions: Member of the Ph.D. Program in Molecular Medicine from 2014-2015; since 2016, she is member of the Scientific Committee of the Dept. of Neurosciences, University of Padova. Her scientific activity focuses on clinical and radiological anatomy, considering the role of adipose tissue in the onset and development of osteoarthritis. Additionally, cooperating with Centre for Mechanics of Biological Materials (CMBM) at the University of Padova, she is active in interdisciplinary research projects based on regenerative medicine and tissue engineering. Regarding Body Donation, Regarding Body Donation, Prof. Macchi is member of the staff of the “Body Donation Program” of the University of Padova (Reference Centre for Body Donation of the Veneto Region) and of the “Inter-department Centre for Teaching on Cadaver - A. Vesalio”. Moreover, she is also responsible for coordination of numerous dissection workshops for surgeons and students of the Medical School. Author of more than 240 in extenso papers with Impact Factor (˃600). Participation in PRIN 2005 and 2007. Head of Research Project of the University of Padova in 2015. Scientific head for Scholarship in Regenerative Medicine and Tissue Engineering (2016 and 2017 from TES - Tissue Engineering and Signaling Foundation onlus). Associate Editor of Surgical and Radiologic Anatomy, European Journal of Anatomy and Journal of the Anatomical Society of India. Speaker and invited speaker at numerous National and International Congresses.
A. Porzionato
Medical Degree (2001 - University of Padova), specialized in “Legal Medicine” (2005 - University of Padova). From October 1, 2005 he is Assistant Professor in Human Anatomy (Institute of Human Anatomy - University of Padova); Ph.D. in Medical, Clinical and Experimental Sciences, Neuroscience curriculum (2009 - University of Padova); from December 1, 2011 he is Associate Professor of Human Anatomy (Institute of Human Anatomy - University of Padova); National Scientific Qualification for Full Professor in Human Anatomy since 2016. Other positions: Member of the Scientific Committee of the Dept. of Molecular Medicine of the University of Padova from 2012-2016; External Reviewer for the Research Grant Council of Hong Kong in 2012; Representative for the School of Medicine in the “Committee for Didactics Quality”; Director of the Course in Aerospace Medicine from 2013-2014; Member of the Ph.D. Program in Molecular Medicine from 2014-2015. Prof. Porzionato is active in many interdisciplinary research projects in the field of regenerative medicine and tissue engineering (development of biological scaffolds and innovative artificial materials), also cooperating with the Centre for Mechanics of Biological Materials (CMBM) at the University of Padova. Other main interests regard experimental models of bronchopulmonary dysplasia, neuroscience (central and peripheral chemoreceptors, neuroanatomy/neuropathology of the brainstem) and clinical anatomy; he is principal investigator of many research projects since 2007. Regarding Body Donation, Prof. Porzionato is member of the staff of the “Body Donation Program” of the University of Padova (Reference Centre for Body Donation of the Veneto Region) and of the “Inter-department Centre for Teaching on Cadaver - A. Vesalio”, also coordinating numerous dissection workshops for surgeons and students of the Medical School. Author of more than 240 in extenso papers with Impact Factor (˃600), speaker at numerous National and International Congresses. Participation in PRIN 2005 and 2007. Head of Research Project of the University of Padova in 2014. Scientific head for Scholarship in Regenerative Medicine and Tissue Engineering (2016 and 2017 from TES - Tissue Engineering and Signaling Foundation onlus). Tutor at numerous workshops of dissection in anatomical and clinical courses for surgeons. Member of the Editorial Board of European Journal of Anatomy, Frontiers in Oncology, Frontiers in Cell and Developmental Biology, Forensic Science and Criminology, Annals of Medical Case Reports - Section: Forensic and Legal Medicine Case Reports. Consultant activities in the field of Forensic Clinical Anatomy.
S Barbon
Ph.D. in Biology and Regenerative Medicine, specialization in Tissue Engineering and Transplantation (2010/2012 - University of Padova). Master Degree in Evolutionary Biology (2007/2009 - University of Padova) and Bachelor Degree in Biology (2004/2007 - University of Padova). From 2013 to 2014, she has been a Post-doc Researcher at T.E.S. Foundation, Padova. In 2015, she worked as Post-doc Researcher at the Department of Molecular Medicine, University of Padova. From 2016 to 2018, she worked as Post-doc Researcher at the Department of Pharmaceutical and Pharmacological Sciences, University of Padova. Since March 2019, she works as Post-doc Researcher et the Department of Neurosciences, University of Padova, in the L.i.f.e.L.a.b. Program of CORIS Veneto. Her scientific activity mainly deals with research programs which combine specific biological science expertises with tissue engineering and regenerative medicine issues with the aim of obtaining biological substitutes that replace or maintain the functionality of human tissues. The experimental work involves the isolation and culture of cells (primary cultures of tissue specific cells and stem cells; immortalized cell lines), studies on growth factors and stem cell differentiation, molecular biology analyses for gene and protein expression, fabrication and repopulation of scaffolds of natural (platelet-rich products, biological matrices) and synthetic (polyvinyl alcohol-based hydrogels) origin and in vivo implantation of the fabricated scaffolds into animal models of tissue damage.